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will not be affected The ideal antibiotic should effectively kill the pathogens responsible for infection and simultaneously cause as little disturbance as possible to the normal ora of the individual But an antibiotic such as this does not exist Yet after oral administration of a four-phage cocktail (Bruttin and Brussow 2005), human volunteers show no alteration whatsoever of normal microbiota It has been demonstrated that it is possible to combine phages and bi dobacteria for the treatment of dysentery in immunosuppressed leukemia patients In this way phages deplete the pathogenic bacteria while bi dobacteria provide a source of new normal ora (Sulakvelidze et al 2001) The side effects due to cell death are the same in antibiotic and phage therapies, but it has been possible to engineer phages for death without lysis (Westwater et al 2003).

To change the default chart type, after selecting a chart from the Change Chart Type dialog box, click the Set as Default Chart button. n

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These engineered phages could offer a very elegant solution for eliminating toxin-producing bacteria such as Clostridium dif cile One of the major criticisms in bacteriophage therapy is the need to identify the causal agent before treatment is begun However, some phages are highly speci c, while others are extremely broad in their host range (Skurnik and Strauch 2006) Recently, the FDA has approved a cocktail of bacteriophages for use against L monocytogenes in ready-to-eat meat and poultry products (Fischetti et al 2006) The application of whole phage particles is not limited to the direct use of phages as active principles; they can also be used as vehicle vaccines in the form of immunogenic peptides to coat proteins or as DNA vaccines (Clark and March 2004).

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Rapid diagnostic kits based on phages have been developed: for example, the fast diagnostic kit for Mycobacterium tuberculosis antibiotic resistance is based on an engineered phage that expressed luciferase and detection with a dental X-ray lm (Riska et al 1999) Drug delivery systems based on viral capsids have also been developed (Kovacs et al 2007) A major pitfall in phage therapy is the pharmacokinetics of the preparations used This is a complex problem because phages are live organisms and are self-replicating The rst consideration to be taken into account is how phages can be stored It has been shown that lyophilized phages show greater stability and activity than liquid preparations (Brussow 2005) An advantage in the formulation of whole phages is that these are quite resistant to environmental conditions, thus allowing a variety of different formulations.

6. Consider input parameters a = 0, b = 1. For M = 2,5,10,50,100 produce graphs containing the pair of functions u, U h .

Although we believe that these methodological changes improve the reliability of the estimated values, they tend to cause lower estimates as compared to the 2003 survey. Thus, the differences in the estimates between 2003 and 2006 may, at least in part, be due to the changes in methodology as opposed to changes in consumers actual experiences. We cannot, therefore, be confident that the difference between the 2003 and 2006 estimates represents an actual drop in the average amount obtained by identity thieves.14

FIGURE 7.28 Choose an option from the Style menu to change the type of strokes the Art History brush applies.

Since the Lown waveform is oscillatory in nature, we know that the circuit is underdamped (~ < I) and Ihe vo ltage appl ied to the patient is of the form

It is dif cult to obtain the exact compositions of the different product preparations and it is also dif cult to know the nal doses inside the organism after colonization of the target bacteria It is therefore mandatory to develop mathematical models that will be able to account for their behavior inside.

Figure 9-7: Digital cameras save metadata with every photograph, providing information about how a picture was taken. (Courtesy of iView Multimedia)

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maybe you are hired to do a cost reduction project, but you end up making some insightful recommendations about organizational structure. You see the big picture and keep your eyes and ears open. You bring clients surprise value and they say, wow, that was really helpful. Available from http:/ /managementconsultingnews.com/newsletter_feb_03.htm#2. 2. Research on client satisfaction is from Ross McManus, Selling and Satisfying the Fortune 1000 in a Post-Enron World, a Survey of Client Satisfaction (November 2003). Available from http:/ /ross-mcmanus.com/release111803 .html. According to senior executives, not understanding their business was the number one reason for terminating professional service providers and consultants. According to the study, one in nine top executives said relationships with their professional services providers and consultants were significantly deteriorating, possibly leading to termination within the next twelve months.

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